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Wydanie 1/2007

Niestabilność mikrosatelitarna w zmianach nowotworowych powiek

Microsatellite Instability in Malignant Eyelids Lesions

Renata Zalewska¹, Ewa Proniewska-Skrętek¹, Zofia Mariak¹, Witold Pepiński², Małgorzata Skawrońska², Jerzy Janica²

¹Klinika Okulistyki Akademii Medycznej w Białymstoku
Kierownik: prof. dr hab. n. med. Zofia Mariak
²Zakład Medycyny Sądowej Akademii Medycznej w Białymstoku
Kierownik: prof. dr hab. n. med. Jerzy Janica

Summary: Analysis of microsatellite instability (MSI) using specific genetic markers is the method of choice in suppressor genes localization assessment responsible for malignant transformation.
The objective of the study was assessment of MSI in eyelid skin tumours.
Material and methods: The studied group comprised 24 patients (12 females and 12 males) aged 43-81 years (average 69.2±9.6) after eyelid tumors surgical resection. Tumor samples had histopathological evaluation using the MSI Analysis System (Promega) containing five fluorescenty-labeled mononucleotide repeat markers: BAT-25, BAT-26, NR-21, NR-24, MONO-27 and two pentanucleotide repeat markers Penta C and Penta D.
Results: Histopathological assessment revealed basocellular carcinoma (4 cases), spinocellular carcioma (2 cases), planoepithelial papilloma (14 cases), keratopapilloma (3 cases), polypus fibrous polyp (1 case). MSI was found only at NR-24 in planoepithelial papilloma in four males aged 43-69 years.
Conclusions: The obtained results suggest limited efficacy of the MSI Analysis System in eyelids malignant lesions identification. Further studies with larger number of patients are needed to elucitade contribution of MSI to development of eyelid skin tumours.

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